Veterinary Research
ISSN: Online 1994-4659ISSN: Print 1993-5412
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Improvement DNA Extraction for Molecular Identification of Eimeria species in Different Age-groups and Breeding Conditions of Chicken in Bacgiang Province, Vietnam
Tran Duc Hoan, Javaid Ali Gadahi and Doan Thi Thao
Page: 34-39 | Received 21 Sep 2022, Published online: 21 Sep 2022
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Abstract
This study aimed to describe the improvement of DNA extraction of coccidial oocysts then DNA extraction used for Polymerase Chain Reaction amplification to identify Eimeria sp. infection in chickens in Bacgiang province of Vietnam. A total of 126 faecal samples were collected from households, semi-commercial and commercial poultry farms in different age-groups. These chickens had no history of vaccination against coccidiosis and had never been given coccidiostat drugs. Oocysts were collected from the microscopically positive samples and DNA was isolated for the PCR (Polymerase Chain Reaction) amplification. The results showed that 87 (77.68%) were positive. The infection rate of E. tenella, E. acervulina, E. brunetti, E. maxima, E. mitis and E. necatrix were 78.16, 70.11, 58.62, 43.68, 28.74 and 26.44%, respectively. Eimeria praecox was not found in this study. So, far in Vietnam, the application of PCR techniques in diagnosis diseases in animal in general and in parasitic diseases in particularly has not been common yet, throughout this study, an important step will be opened the new tendency in improvement of DNA extraction and molecular diagnosis animal diseases in stead of conventional methods.
How to cite this article:
Tran Duc Hoan, Javaid Ali Gadahi and Doan Thi Thao. Improvement DNA Extraction for Molecular Identification of
Eimeria species in Different Age-groups and
Breeding Conditions of Chicken in Bacgiang Province, Vietnam.
DOI: https://doi.org/10.36478/vr.2014.34.39
URL: https://www.makhillpublications.co/view-article/1993-5412/vr.2014.34.39