@article{MAKHILLVR20147428040,
    title = {Improvement DNA Extraction for Molecular Identification of
Eimeria species in Different Age-groups and
Breeding Conditions of Chicken in Bacgiang Province, Vietnam},
    journal = {Veterinary Research},
    volume = {7},
    number = {4},
    pages = {34-39},
    year = {2014},
    issn = {1993-5412},
    doi = {vr.2014.34.39},
    url = {https://makhillpublications.co/view-article.php?issn=1993-5412&doi=vr.2014.34.39},
    author = {Tran,Javaid and},
    keywords = {Eimeria sp.,Improvement,chicken,bacgiang province,vietnam},
    abstract = {This study aimed to describe the improvement of DNA extraction of coccidial oocysts then DNA
extraction used for Polymerase Chain Reaction amplification to identify Eimeria sp. infection in chickens in
Bacgiang province of Vietnam. A total of 126 faecal samples were collected from households, semi-commercial
and commercial poultry farms in different age-groups. These chickens had no history of vaccination against
coccidiosis and had never been given coccidiostat drugs. Oocysts were collected from the microscopically
positive samples and DNA was isolated for the PCR (Polymerase Chain Reaction) amplification. The results
showed that 87 (77.68%) were positive. The infection rate of E. <i>tenella</i>, E. <i>acervulina</i>, E. <i>brunetti</i>, E. <i>maxima</i>,
E. mitis and E. necatrix were 78.16, 70.11, 58.62, 43.68, 28.74 and 26.44%, respectively. Eimeria praecox was
not found in this study. So, far in Vietnam, the application of PCR techniques in diagnosis diseases in animal
in general and in parasitic diseases in particularly has not been common yet, throughout this study, an important
step will be opened the new tendency in improvement of DNA extraction and molecular diagnosis animal
diseases in stead of conventional methods.}
    }