Research Journal of Biological Sciences
ISSN: Online 1993-6087ISSN: Print 1815-8846
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Amplification of Bone Morphogenetic Protein 2 (BMP2) in Avian Genome and Cloning it into Pichia Pink α-HC and Express it in Pichia pastoris
Richard The Swee Aun, Jalila Abu, Abdul Rahman Omar and Jayaraj Vijaya Kumaran
Page: 124-129 | Received 21 Sep 2022, Published online: 21 Sep 2022
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Abstract
This study was conducted to detect the presence of Bone Morphogenetic Protein 2 (BMP2) gene within the avian genome and cloned it into Pichia pastoris for future usage. DNA was extracted from feathers and preceded to BMP2 gene detection via Polymerase Chain Reaction (PCR). Agarose gel presented positive result for BMP2 gene amplification. Then BMP2 gene was then cloned into pCR2.1-TOPO plasmid and sent for sequencing to further confirm the presence of BMP2 gene. Followed by, inserted the BMP2 gene into Pichia Pink α-HC then allowed the BMP2 gene to express the BMP2 protein with Pichia pastoris expression System. SDS-PAGE results shown presence of the BMP2 protein and it was further confirm using Chromogenic Development method.
How to cite this article:
Richard The Swee Aun, Jalila Abu, Abdul Rahman Omar and Jayaraj Vijaya Kumaran. Amplification of Bone Morphogenetic Protein 2 (BMP2) in Avian Genome and Cloning it into
Pichia Pink α-HC and Express it in Pichia pastoris.
DOI: https://doi.org/10.36478/rjbsci.2020.124.129
URL: https://www.makhillpublications.co/view-article/1815-8846/rjbsci.2020.124.129