TY  - JOUR
T1  - Amplification of Bone Morphogenetic Protein 2 (BMP2) in Avian Genome and Cloning it into
Pichia Pink &#945;-HC and Express it in <i>Pichia pastoris</i>
AU - Abu, Jalila AU - The Swee Aun, Richard AU - Rahman Omar, Abdul AU - Vijaya Kumaran, Jayaraj 
JO  - Research Journal of Biological Sciences
VL  - 15
IS  - 5
SP  - 124
EP  - 129
PY  - 2020
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2020.124.129
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2020.124.129
KW  - Bone Morphogenetic Protein 2
KW  -BMP2
KW  -Demineralized Bone Matrix
KW  -DBM
KW  -Pichia pastoris
KW  -Protein expression and PCR
AB  - This study was conducted to detect the
presence of Bone Morphogenetic Protein 2 (BMP2) gene
within the avian genome and cloned it into <i>Pichia pastoris</i>
for future usage. DNA was extracted from feathers and
preceded to BMP2 gene detection via Polymerase Chain
Reaction (PCR). Agarose gel presented positive result for
BMP2 gene amplification. Then BMP2 gene was then
cloned into pCR2.1-TOPO plasmid and sent for
sequencing to further confirm the presence of BMP2
gene. Followed by, inserted the BMP2 gene into Pichia
Pink &#945;-HC then allowed the BMP2 gene to express the
BMP2 protein with <i>Pichia pastoris</i> expression System.
SDS-PAGE results shown presence of the BMP2 protein
and it was further confirm using Chromogenic
Development method.
ER  -