Research Journal of Biological Sciences
ISSN: Online 1993-6087ISSN: Print 1815-8846
Abstract
Lipid Transfer Protein encoded by LTP gene in plants. This is a GC rich gene. These base causes that designed primers for LTP gene produce many secondary structures in annealing step of PCR and eventually does not produce a suitable product. Substances such as betaine, Dimethyl Sulfoxide (DMSO) and Ammonium Sulfate (AMS) can to eliminate this obstacle. In this study, the gradient of each material was placed in the PCR reaction and ultimately the optimum concentration for any of this material was obtained. In between, 4.5 μL betaine (5M), 2.25 μL DMSO (10%) and 2.25 μL AMS in the overall volume of 25 μL of PCR mixture were reported optimal concentrations.
How to cite this article:
Mohammad Karimian, Mehran Miroliaie, Kamran Ghaedi and Zohreh Zahraie. Improve the Amplification of LTP (Lipid Transfer Protein) Gene.
DOI: https://doi.org/10.36478/rjbsci.2010.802.804
URL: https://www.makhillpublications.co/view-article/1815-8846/rjbsci.2010.802.804