TY  - JOUR
T1  - Improve the Amplification of <I>LTP </I>(Lipid Transfer Protein) Gene
AU - Karimian, Mohammad AU - Miroliaie, Mehran AU - Ghaedi, Kamran AU - Zahraie, Zohreh 
JO  - Research Journal of Biological Sciences
VL  - 5
IS  - 12
SP  - 802
EP  - 804
PY  - 2010
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2010.802.804
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2010.802.804
KW  - Gradient PCR
KW  -secondary structures
KW  -betaine
KW  -dimethyl sulfoxide
KW  -ammonium sulfate buffer
KW  -Iran
AB  - Lipid Transfer Protein encoded by <I>LTP </I>gene in plants. This is a GC rich gene. These base causes that designed primers for <I>LTP</I> gene produce many secondary structures in annealing step of PCR and eventually does not produce a suitable product. Substances such as betaine, Dimethyl Sulfoxide (DMSO) and Ammonium Sulfate (AMS) can to eliminate this obstacle. In this study, the gradient of each material was placed in the PCR reaction and ultimately the optimum concentration for any of this material was obtained. In between, 4.5 &#956;L betaine (5M), 2.25 &#956;L DMSO (10%) and 2.25 &#956;L AMS in the overall volume of 25 &#956;L of PCR mixture were reported optimal concentrations.
ER  -