Jie  Sun, Jinfu  Wang, Daquan  Li, Shuang  Li, Zongsheng  Zhao, Yourong  Ye, 
    In vitro Effects of Sex Hormones on the Proliferation of
Endometrial Cells Isolated from Laying Hens,
    Veterinary Research,
    Volume 7,Issue 2,
    2014,
    Pages 13-19,
    ISSN 1993-5412,
    vr.2014.13.19,
    (https://makhillpublications.co/view-article.php?doi=vr.2014.13.19)
    Abstract: Researchers investigated the effects of Estrogen (E<sub>2</sub>) and Progesterone (P<sub>4</sub>) on the morphology,
growth and proliferation of chicken endometrial cells <i>in vitro</i>. Type I collagenase digestion was used to isolate
endometrial epithelial and stromal cells and the cells were identified by immunohistochemistry. MTT assays
(MTT, [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide]) were performed to analyze the effects
of the sex hormones on the proliferation of the endometrial cells in vitro and growth curves were constructed
to demonstrate the growth rules of endometrial cells cultured <i>in vitro</i>. The epithelial and stromal cells tested
positive for keratin CK18 and vimentin, respectively, in the immunohistochemical analysis. The MTT
experiments showed that the growth rate of the chicken endometrial cells began to increase logarithmically on
day 2, reaching the plateau phase on day 6 or 7. The endometrial epithelial cells treated with 100 nM E<sub>2</sub> and/or
100 nM P<sub>4</sub> proliferated at a significantly higher rate p&lt;0.05) than the control cells. However, although, the
stromal cells treated with 100 nM P4 proliferated at a significantly higher rate (p&lt;0.05) than the control cells,
the E<sub>2</sub>-treated endometrial stromal cells did not.
    Keywords: epithelium cell;laying hen;proliferation in vitro;Endometrium;stromal cell;sex hormone