Tao  Jiang, Liangcheng  Chen, Xiao  Li, Qi  Wang, Yu  Li, Kevin  Marley, Yongqiang  Wang, 
    Early Detection of <i>Aspergillus fumgitus</i> in a High Risk Group of Dairy Cattle using Nested and
Real-Time Quantitative PCR,
    Research Journal of Dairy Sciences,
    Volume 8,Issue 4,
    2014,
    Pages 34-40,
    ISSN 1993-5277,
    rjdsci.2014.34.40,
    (https://makhillpublications.co/view-article.php?doi=rjdsci.2014.34.40)
    Abstract: Early detection of Aspergillus fumigatus
(<i>A. fumigatus</i>) may facilitate early diagnosis and
prevention of Invasive Aspergillosis (IA) in dairy cows.
This study used a nested PCR and real-time quantitative
PCR method to measure A. fumigatus in feed, tissue and
blood samples collected from domestic dairy farms in
China. In total, 396 feed samples from across China
including corn, soybean meal, cotton seed meal, wheat
mill run, distiller&#146;s dried grains, corn and grass silage and
Total Mixed Ration (TMR) were tested. Of these, 95%
contained detectable levels of A. fumigatus. Blood and
tissue samples from the GI tract of 7 unhealthy cows all
tested positive for A. fumigatus. The analytical sensitivity
of the PCR method was&#60;1 fg/assay.
    Keywords: Aspergillus fumgitus;feed;real-time quantitative;PCR;form