Qinghua  Chen, Xinghua  Pan, Xiangqing  Zhu, Xuemin  Cai, 
    Differentiation Peripheral Blood Mononuclear Cells into Functional Hematic-Like Cells by Hematopoietic Remodeling,
    Research Journal of Medical Sciences,
    Volume 7,Issue 5-6,
    2013,
    Pages 148-155,
    ISSN 1815-9346,
    rjmsci.2013.148.155,
    (https://makhillpublications.co/view-article.php?doi=rjmsci.2013.148.155)
    Abstract: The shortage of bone marrow donors and the high risk of allogenic 
  transplant rejection have prompted investigation into alternative therapeutic 
  approaches for patients with aplastic anemia. Reconstitution of hematopoietic 
  function using stem cells derived from cord blood is a promising alternative 
  which however, the limited number of stem cells obtained restricts clinical 
  application. In the earlier study, researchers demonstrated the epigenetic reprogramming 
  of mouse skin fibroblasts into multipotent cells using a transient <I>in vitro</I> 
  method. After transient reprogramming, cells expressed reprogramming factors 
  (Oct-3/4, Nanog and Sox-2) and formed teratomas in nude mice. In this study, 
  PBMCs from aplastic anemia patients were reprogrammed using the transient <I>in 
  vitro</I> method. Reprogrammed cells (iMS cells) were capable of differentiating 
  into hematopoietic cells; iMS cells transplanted into lethally irradiated mice 
  differentiated into bone marrow and splenic cells. Results indicate that reprogrammed 
  PBMCs are multipotent and can restore hematopoietic function.
    Keywords: Peripheral blood mononuclear cells;hematopoietic stem cells;multipotent stem cells;fish oocyte extracts;reprogramming