Marco Antonio Becerril Flores  , Salazar Schettino Paz Maria  , Ramirez Zamudio Lina  , 
    Analysis of Variability of Clones and Subclones of <I>Trypanosoma cruzi </I>Derived from Mexican Strains by the Behavior in Mice and Culture Cells,
    Research Journal of Medical Sciences,
    Volume 2,Issue 4,
    2008,
    Pages 166-174,
    ISSN 1815-9346,
    rjmsci.2008.166.174,
    (https://makhillpublications.co/view-article.php?doi=rjmsci.2008.166.174)
    Abstract: To better understand the biological heterogeneity of behavior of <I>Trypanosoma cruzi</I> strains isolated from Mexico, 6 strains of <I>T. cruzi </I>were cloned by the Miles’ method (drops of suspension with parasites were diluted in phosphate-buffered saline solution and inoculated to mice).Virulence and infectivity of 10 or 11 clones derived from each strain were determined in female Balb/c mice and Vero culture cells, respectively. Variability of clones was determined by Tukey F statistic test. Only 1 strain, T5, showed interclonal variability and its clones were subcloned by the same method; they showed similar behavior as their parental clones. Clones increased the virulence or had the same behavior after maintaining them for 1 year in mice. Seven clones were eliminated and the virulence of the 4 remaining clones was attenuated when they were maintained in LIT axenic culture for 1 year. The study showed that the strains of <I>T. cruzi</I> in Mexico are either monoclonal or polyclonal and the clones could be eliminated or selected from the environment along time; therefore it is possible to observe that the behavior of <I>T. cruzi</I> strains can change when they are maintained in laboratory for several years.
    Keywords: Variability;clones;T. cruzi;mice and culture cells