Li Jilun, Nining Hong, Tian Jiesheng,
Heteroexpression of Bacteriophage T7 Lysozyme in Magnetospirillum gryphiswaldense
Facilitates Large-Scale Extraction of Magnetosomes,
Research Journal of Biological Sciences,
Volume 12,Issue 1,
2017,
Pages 6-12,
ISSN 1815-8846,
rjbsci.2017.6.12,
(https://makhillpublications.co/view-article.php?doi=rjbsci.2017.6.12)
Abstract: To improve the efficiency of magnetosome extraction, a lysozyme expressing strain of
Magnetospirillum gryphiswaldense MSR-1, named MLE was constructed. Compared to wild type, MLE
showed no significant differences in cell growth and magnetosome formation. Colorimetric monitoring and
transmission electron microscopy confirmed that MLE cells could be disrupted more effectively following freeze
thaw treatment. Importantly, extraction of magnetosomes from the engineered strain could be easily performed
on a large scale.
Keywords: Extraction;lysozyme;magnetosomes;Magnetospirillum gryphiswaldense;China