M.A.  Khaled, A.  Jalila, H.  Kalthum, M.  Noordin, W. Asma  Saleh, 
    Collagen-Calcium Alginate Film Dressing with Therapeutic Ultrasound to Treat Open Wound in Rats,
    Research Journal of Biological Sciences,
    Volume 9,Issue 2,
    2014,
    Pages 57-61,
    ISSN 1815-8846,
    rjbsci.2014.57.61,
    (https://makhillpublications.co/view-article.php?doi=rjbsci.2014.57.61)
    Abstract: About 24 healthy female Sprague-Dawley rats weighing between 300-350 g were 
  used in this study over a 20 day period. They were allocated randomly into 4 
  groups of 6 animals each. After the creation of 2x2 cm open wound, group 1 was 
  control treated with Gentamycin ointment. Groups 2-4 were treated with Therapeutic 
  ultrasound massage, collagen-calcium alginate film and collagen-calcium alginate 
  film with therapeutic ultrasound. On application, the collagen-calcium alginate 
  film with therapeutic ultrasound was well accepted by the animals without any 
  adverse reaction. Mean percentage of wound contraction were significantly better 
  in group 4 (p<0.05). Faster epithelialization was also seen in the collagen-calcium 
  alginate film with therapeutic ultrasound treated group as compared to the other 
  groups. Collagen is a biocompatible protein that does not interfere with the 
  body’s normal immunologic response and can be used in non-healing chronic 
  wounds which require a trigger to stimulate the normal healing process. In extensive 
  wounds when there is lack of autologous tissue, biomaterials like collagen-calcium 
  alginate may be beneficial and can be used.
    Keywords: Ultrasound;collagen;calcium alginate;wound;biomaterials;tissues