S.H. Anvari  , M. Sattari  , M. Forozandehe Moghadam  , S.H. Najar Peerayeh  , A.A. Imanee Fouladi  , 
    Detection of <I>Staphylococcus aureus</I> Enterotoxins A to E from Clinical Sample by PCR,
    Research Journal of Biological Sciences,
    Volume 3,Issue 8,
    2008,
    Pages 826-829,
    ISSN 1815-8846,
    rjbsci.2008.826.829,
    (https://makhillpublications.co/view-article.php?doi=rjbsci.2008.826.829)
    Abstract: <I>Staphylococcus aureus</I> is one of the most significant pathogens causing nosocomial and community acquired infections. Among the secreted Staphylococcal virulence factors, there is a growing list of enterotoxins which can induce gastroenteric syndrome and toxic shock syndrome. Here we used PCR for the detection of genes  encoding  Staphylococcal  enterotoxins  A,  B,  C,  D,  E  (SEA, SEB, SEC1, SED and SEE) of <I>S. aureus.</I> SEA-SEE were selected because they are 5 classically Described enterotoxins of <I>S. aureus</I> and because they were detected by Latex agglutination. We investigated 50 isolates of <I>S. aureus</I> drived from scar of patients. The presence of enterotoxin genes was found in 37 (74%) of total number of 50 isolates, for one or more enterotoxin genes. The PCR is more sensitive because it offers the possibility for determining enterotoxin as on a genotypic basis.
    Keywords: Staphylococcus aureus;enterotoxins;A-E;clinical sample;PCR