Nagi A. AL-Haj  , Mariana N. Shamsudin  , Raha A. Rahim  , Zamri Ishak  , 
    Development of a PCR Primer and a Marker Band for Detection of <I>E. coli</I> from Various Sources Based on Arbitrary Primer Set,
    Research Journal of Biological Sciences,
    Volume 3,Issue 11,
    2008,
    Pages 1327-1332,
    ISSN 1815-8846,
    rjbsci.2008.1327.1332,
    (https://makhillpublications.co/view-article.php?doi=rjbsci.2008.1327.1332)
    Abstract: Although, PCR methods aimed on the detection of genes associated with the pathogenicity of <I>Escherichia coli</I> have been reported, tests allowing the direct identification of this serotype are rare. In this study the Random Amplified Polymorphic DNA (RAPD) fingerprinting technique allowed genetic diversity assessment of 25 <I>E. coli</I> isolates of various sources. A highly significant finding from the DNA fingerprinting is the display of a predominant band at a size of 308 bp when arbitrary OPAE-10 primer was used. After sequencing this fragment primer called <I>secD</I> was designed to be used as PCR primer. <I>secD</I> primer pairs was highly specific to detect all isolates including <I>E. coli</I> O157: H7.
    Keywords: Random Amplified Polymorphic DNA (RAPD);uropathogenic E. coli (UPEC);Neonatal Meningitis-associated E. coli (MNEC);University Putra Malaysia (UPM);primer;sequence