TY  - JOUR
T1  - <I>In vitro</I> and <I>in vivo</I> Antiplasmodial Activities of Stem Bark Extracts of <I>Garcinia parvifolia</I> Miq (Guttiferae)
AU - , Syamsudin AU - , Soesanto Tjokrosanto AU - , Subagus Wahyuono AU - , Darmono AU - , Mustofa 
JO  - International Journal of Tropical Medicine
VL  - 2
IS  - 2
SP  - 41
EP  - 44
PY  - 2007
DA  - 2001/08/19
SN  - 1816-3319
DO  - ijtmed.2007.41.44
UR  - https://makhillpublications.co/view-article.php?doi=ijtmed.2007.41.44
KW  - Plasmodium falciparum
KW  -Garcinia parvifolia
KW  -traditional plant
KW  -antimalarial drug
KW  -in vitro
KW  -in vivo
KW  -Plasmodium berghei
AB  - <I>In vitro </I>and <I>in vivo</I> antiplasmodial activities of stem barks extracts of <I>Garcinia parvifolia</I> Miq, medicinal plant traditionally used to treat malaria in Indonesia, have been evaluated. The IC<SUB>50</SUB> value of extracts was determined. Extracts of this plant (n-hexane, ethylacetate and n-buthanol extracts) were tested <I>in vitro</I> for their antiplasmodial activity on 2 strains of <I>Plasmodium falciparum</I>, FCR-3 (chloroquine-resistant strain) and 3D7 (chloroquine-sensitive strain) using a visual method. Cytotoxicity of these extracts were performed on HeLa cells <I>in vitro</I> using MTT method. The <I>in vivo</I> antiplasmodial testing used a standard 4-days test on <I>P. berghei</I> infected  mice  and  acute  toxicity  testing  on mice were also conducted. The IC<SUB>50</SUB> values of the plant extracts were in the range of 4,83-40,10 µg mL <SUP>1</SUP>. Significant <I>in vitro</I> antiplasmodial activity and higher selective toxicity were observed by n-hexane extract (IC<SUB>50</SUB> = 4,11 &micro;g mL <SUP>1</SUP> and C/A = 48,23). The n-hexane extract was the most active <I>in vivo</I> against <I>P. berghei</I> (ED<SUB>50</SUB> = 19,95 mg kg <SUP>1</SUP>BW) and least toxic in mice (LD<SUB>50</SUB>= 1060,47 mg kBW <SUP>1</SUP>). These results suggest that n-hexane extract is promising extract for further investigation for new antimalarial agents.
ER  -