TY  - JOUR
T1  - Heteroexpression of Bacteriophage T7 Lysozyme in <I>Magnetospirillum gryphiswaldense</I>
Facilitates Large-Scale Extraction of Magnetosomes
AU - Jilun, Li AU - Hong, Nining AU - Jiesheng, Tian 
JO  - Research Journal of Biological Sciences
VL  - 12
IS  - 1
SP  - 6
EP  - 12
PY  - 2017
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2017.6.12
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2017.6.12
KW  - Extraction
KW  -lysozyme
KW  -magnetosomes
KW  -Magnetospirillum gryphiswaldense
KW  -China
AB  - To improve the efficiency of magnetosome extraction, a lysozyme expressing strain of
<I>Magnetospirillum gryphiswaldense</I> MSR-1, named MLE was constructed. Compared to wild type, MLE
showed no significant differences in cell growth and magnetosome formation. Colorimetric monitoring and
transmission electron microscopy confirmed that MLE cells could be disrupted more effectively following freeze
thaw treatment. Importantly, extraction of magnetosomes from the engineered strain could be easily performed
on a large scale.
ER  -