TY  - JOUR
T1  - Investigating Immune Responses to Putative Diabetogenic Modified Bovine Proteins Subjected to Microwave Heating among Young Jordanian Children with Type 1 Diabetes Mellitus
AU - Al-Domi, H.A. AU - Jaradat, Z.W. AU - Jones, R.M. AU - Ababneh, Q.O. 
JO  - Research Journal of Biological Sciences
VL  - 5
IS  - 8
SP  - 521
EP  - 528
PY  - 2010
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2010.521.528
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2010.521.528
KW  - BSA
KW  -insulin
KW  -immunogenicity
KW  -diabetes
KW  -autoimmune
KW  -protein
AB  - The objective of this study was to examine the effect of household Microwave Heating (MWH) on the immunogenicity of a putative diabetogenic protein among young Jordanian diabetic children. Bovine serum albumin (1 mg mL<SUP>-1</SUP>) was modified at physiological pH (7.4) by incubating with equal volume of bovine insulin (1 mg mL<SUP>-1</SUP>) at 37&deg;C for 24 h. The developed modified Bovine Serum Albumin with Bovine Insulin (mBI-BSA) was subjected to MWH for 30, 60, 90 and 120 sec, cooled immediately to 0&deg;C prior to analysis. Blood samples were collected from 76 diabetic and healthy children. Sera were tested for presence of antibodies against mBI-BSA by ELISA. Anti-BSA, anti-mBI-BSA and anti-microwave heated mBI-BSA antibodies were detected in diabetic and healthy children. The difference between groups was insignificant (p&lt;0.05). Anti-mBI-BSA antibodies were significantly higher than that produced against native BSA (p&gt;0.05) with no significant difference between groups. The difference in sera and saliva titers against mBI-BSA between diabetic children and their control was insignificant (p&gt;0.05).The effect of MWH with different exposure times on anti-mBI-BSA was nearly similar to that produced against un heated mBI with no significant difference between groups.
ER  -