TY  - JOUR
T1  - Genes Expression in Biosynthesis Lipopolysaccharide of <I>Burkholderia pseudomallei</I> the Causative Agent of Melioidosis
AU - Al-Haj, Nagi A. AU - Suang, Lai L. AU - Shamsudin, Mariana N. AU - Abdullah, Rasedee AU - Mohamed, Rahmah AU - Sekawi, Zamberi 
JO  - Research Journal of Biological Sciences
VL  - 4
IS  - 6
SP  - 668
EP  - 674
PY  - 2009
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2009.668.674
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2009.668.674
KW  - Lipopolysaccharides
KW  -melioidosis
KW  -Burkholderia pseudomallei
KW  -expression
KW  -restriction enzyme digestion
KW  -Western blot
AB  - <I>Burkholderia pseudomallei</I> is the causative agent of melioidosis, a serious disease of man and animals. The high mortality of <I>B. pseudomallei</I> infections may cause by Lipopolysaccharides (LPS), an endotoxin. The biosynthesis of LPS is complex comprising three components, lipid A, core oligosaccharide and O-specific antigen. In the current study was designed to further elucidate genes involved in the biosynthesis pathway of LPS in melioidosis agent followed with selected gene product expression with essential function for survival and virulence melioidosis agent. Expression of <I>Bplps0013</I>/<I>lpxA</I> and <I>Bplps0007</I>/<I>rfaF</I> successful expressed the entire proteins in 2 h with sizes of approximately 29 kDa and 43.7 kDa, respectively. The baseline information provided through the present research can be a preliminary approach towards the development of effective therapeutics against melioidosis.
ER  -