TY  - JOUR
T1  - Chitosanase Production by a New Bacterial Sources
AU - , A.V. Charles-Rodriguez AU - , J.E. Mauricio-Benavides AU - , Y. Garza-Garcia AU - , C.N. Aguilar AU - , R. Rodriguez-Herrera AU - , J. Rodriguez AU - , J.C. Contreras-Esquivel 
JO  - Research Journal of Biological Sciences
VL  - 3
IS  - 8
SP  - 957
EP  - 963
PY  - 2008
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2008.957.963
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2008.957.963
KW  - Chitosanolytic activity
KW  -chitosan-oligosaccharides
KW  -Aeromonas sp.
AB  - Microbial marine enzymes can offer novel biocatalysts with properties such as high salt tolerance, hypoerthermostability, barophilicity and a more simple approach in large scale cultivation. Chitosanases are enzymes with importance as low molecular weight chitosans and chitosan-oligomers producers, which show innumerable applications in various fields, such as the biomedical, pharmaceutical, agricultural, biotechnological and food industry fields. However, the use of chitosanase is limited due to of its high-cost implications and potential unavailability in bulk quantities. In this study the production of bacterial chitosanases was evaluated in new isolated sources from Valley of Cuatrocienegas, Coahuila, Mexico. Specific culture media using chitosan-oligomers was used as a single source of carbon. Under these conditions, <I>Aeromonas </I>sp.<I> </I>produced 501 U L <SUP>-1</SUP> of chitosanase activity and 220 U L <SUP>-1</SUP> for <I>Bacillus cereus</I>, respectively.<I> </I>The crude chitosanase activity of culture supernatant was maximal on the 150 h of culture. Below 40ºC, viscosity kinetics have indicated that chitosan is efficiently hydrolyzed into chitosan-oligosaccharides by <I>Aeromonas sp </I>and <I>Bacillus cereus</I> in a more rapid manner<I>. </I>SDS-PAGE of enzymatic extract of <I>Aeromonas </I>sp.<I> </I>has indicated a single band of ~ 20 kDa.
ER  -