TY - JOUR T1 - Detection of Staphylococcus aureus Enterotoxins A to E from Clinical Sample by PCR AU - , S.H. Anvari AU - , M. Sattari AU - , M. Forozandehe Moghadam AU - , S.H. Najar Peerayeh AU - , A.A. Imanee Fouladi JO - Research Journal of Biological Sciences VL - 3 IS - 8 SP - 826 EP - 829 PY - 2008 DA - 2001/08/19 SN - 1815-8846 DO - rjbsci.2008.826.829 UR - https://makhillpublications.co/view-article.php?doi=rjbsci.2008.826.829 KW - Staphylococcus aureus KW -enterotoxins KW -A-E KW -clinical sample KW -PCR AB - Staphylococcus aureus is one of the most significant pathogens causing nosocomial and community acquired infections. Among the secreted Staphylococcal virulence factors, there is a growing list of enterotoxins which can induce gastroenteric syndrome and toxic shock syndrome. Here we used PCR for the detection of genes encoding Staphylococcal enterotoxins A, B, C, D, E (SEA, SEB, SEC1, SED and SEE) of S. aureus. SEA-SEE were selected because they are 5 classically Described enterotoxins of S. aureus and because they were detected by Latex agglutination. We investigated 50 isolates of S. aureus drived from scar of patients. The presence of enterotoxin genes was found in 37 (74%) of total number of 50 isolates, for one or more enterotoxin genes. The PCR is more sensitive because it offers the possibility for determining enterotoxin as on a genotypic basis. ER -