TY  - JOUR
T1  - Molecular Characterization of Horseshoe Crab Anti-Lipopolysaccharide Factor C-Peptide for Hybridization-Based Detection Method of Gram Negative Bacteria
AU - , Nagi A. Al-Haj AU - , Mariana N. Shamsudin AU - , Raha A. Rahim AU - , Zamri Ishak 
JO  - Research Journal of Biological Sciences
VL  - 3
IS  - 12
SP  - 1392
EP  - 1397
PY  - 2008
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2008.1392.1397
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2008.1392.1397
KW  - Gram negative bacteria
KW  -lipopolysaccharide
KW  -horseshoe crab
KW  -factor C anti-LPS
KW  -polymerase chain reaction
KW  -sequencing
KW  -dot blot hybridization
AB  - Recent advances in molecular techniques have revolutionized the detection of microorganism. The development of a molecular-based technique for detection of the three different targets of Enterbacteriacae was undertaken. Primer and probe were designed based on specific pepted of novel hemolymph protein of horseshoe crabs (Factor C anti-LPS) <I>Tachypleus tridentatus</I> that is believed to be involved in the binds to the lipopolysaccharide of <I>Escherichia coli</I>,<I> Salmonella</I> and <I>Vibrio cholerae</I>. The aim of our study the exploit part of cell wall polysaccharide in the development of improved detection method based on molecular approaches. In the gene detection assay, Lipopolysaccharide gene of <I>Salmonella</I>,<I> V. cholera</I> and <I>E. coil </I>were hybridized to anti-LPS factor gene found in the  biolysate  of  the  marine  animals.  The  wzm  and  wzt  genes encoding O-polysaccharide genes were amplified in these pathogens and the LPS factor C were amplified from the marine lysate. Development of a  PCR-based  technique    for  detection  of  the  food-borne  pathogens  particularly <I>Sa Salmonella</I>,<I> V. cholera</I> and <I>E. coil</I> were achieved. Thus rapid, sensitive and reliable techniques for the detection of food-borne pathogens developed.
ER  -