@article{MAKHILLVR202114328068,
    title = {Evaluation of Blastocyst Rates of Immature Bovine Oocytes Collected by Ovum Pick up (OPU)
and Slaughterhouse},
    journal = {Veterinary Research},
    volume = {14},
    number = {3},
    pages = {27-33},
    year = {2021},
    issn = {1993-5412},
    doi = {vr.2021.27.33},
    url = {https://makhillpublications.co/view-article.php?issn=1993-5412&doi=vr.2021.27.33},
    author = {Sakine,Dursun,Mustafa,Muhammed,Ömer,Vahit,Ayşe,Maide and},
    keywords = {Embryo,oocyte,ovum pick up,post-mortem},
    abstract = {This study was aimed to produce <i>in-vitro</i>
embryos massively and at a lower cost. For this purpose,
it was aimed to determine and compare the blastocyst
growth rates of oocytes collected from slaughterhouse and
by Ovum-Pick up (OPU) method. The animal material to
be used in the OPU application of the study consisted of
10 Holstein breed heifers 15-25 months-old. A total
of 51 times OPU was applied to the animals. In the
postmortem study, 52 ovaries belonging to 26 Holstein
cows obtained from local slaughterhouses were used.
After the obtained oocytes were evaluated according to
their quality, they were taken to maturation, fertilization
and culture stages respectively. In the presented study,
total of 359 oocytes were collected in 51 OPU
applications. The cleavage rate was 85.07% and the rate
of blastocyst growth was 24,63%. Collected from the
slaughterhouse, 325 oocytes were obtained from 52
ovaries. The cleavage rate was determined as 94.19% and
the rate of reaching blastocyst was determined as 25.73%.
As a result, it was observed that there was no statistical
difference in blastocyst development rates (p>0.05). In the
production of <i>in vitro</i> embryos, it has been concluded that
by obtaining post-slaughtering ovaries of females with
high genetic value, the loss of genetic material can be
reduced by producing their embryos at cheaper costs
(cost-effective). It is suggested that <i>in-vitro</i> embryo
production can be carried out massively and at a cheaper
cost from slaughtered female animals and material with
average or above genetic characteristics can be evaluated.}
    }