@article{MAKHILLRJBS20094510917, title = {Cloning and Sequencing of Strawberry Chitinase Gene CHI2}, journal = {Research Journal of Biological Sciences}, volume = {4}, number = {5}, pages = {624-628}, year = {2009}, issn = {1815-8846}, doi = {rjbsci.2009.624.628}, url = {https://makhillpublications.co/view-article.php?issn=1815-8846&doi=rjbsci.2009.624.628}, author = {Zhang Yong,Tang Hao-ru,Luo Ya,Jiang Hao and}, keywords = {Strawberry,chitinase gene,cloning,sequencing,amino acid,CHI2}, abstract = {Two genes fragments from cultivated strawberry (Fragaria x ananassa cv. Toyonaka and cv. Akihime) genomic DNA were isolated by PCR amplification. The study showed that these genes were 1575 bp in length and contained a 988 bp Open Reading Frome (ORF) and 2 extron and 1 intron. The homology of nucleotide and deduced amino acid were 99.05 and 98.56%, respectively. Sequecing revealed that the 2 genes encoded a class chitinase. BLAST analysis showed that the homology of deduced amino acid between strawberry and other species’s CHI2 gene were about 50%. The homology tree showed that it obviously distinguished from the others.} }