@article{MAKHILLRJBS20094910989, title = {Synthesis and Antibacterial Activity of Silver/Montmorillonite Nanocomposites}, journal = {Research Journal of Biological Sciences}, volume = {4}, number = {9}, pages = {1032-1036}, year = {2009}, issn = {1815-8846}, doi = {rjbsci.2009.1032.1036}, url = {https://makhillpublications.co/view-article.php?issn=1815-8846&doi=rjbsci.2009.1032.1036}, author = {Mansor Bin,Kamyar,Majid,Wan Md Zin,Nor Azowa,Azizah and}, keywords = {Silver nanoparticles,montmorillonite,nanocomposite,antibacterial activity,powder x-ray diffraction,muller-hinton agar}, abstract = {Silver nanoparticles (Ag-NPs) were successfully synthesized into the interlayer space of Montmorillonite (MMT) by chemical reduction method. AgNO3 and NaBH4 were used as a silver precursor and reducing agent, respectively. The properties of Ag/MMT nanocomposites were studied as a function of the AgNO3 concentration. The UV-vis spectra of synthesized Ag-NPs showed that the intensity of the maximum wavelength of the plasmon peaks increased with increasing AgNO3 concentration. The crystalline structure of the Ag-NPs and basal spacing of MMT and Ag/MMT were also studied by Powder X-Ray Diffraction (PXRD). The antibacterial activity of Ag-NPs was investigated against gram-negative bacteria (Escherichia coli, Escherichia coli O157:H7 and K. pneumonia) and gram-positive bacterium (Staphylococcus aureus) by disk diffusion method using Muller-Hinton Agar (MHA) at different sizes of Ag-NPs. The smaller Ag-NPs were found to have significantly higher antibacterial activity. These results showed that Ag-NPs can be used as effective growth inhibitors in different biological systems, making them applicable to medical applications such as in surgical devices.} }